Where is the iPhone backup file located on my Mac computer? I created the backup with iTunes for Mac.

If you used iTunes for Mac to create a backup of your iPhone, the backup file will be located in the "/Users/{userid}/Library/Application Support/MobileSync/Backup" folder.

A sub-folder will be created for each backup. For example, here is an iPhone backup sub-folder:

fyicenter$ cd ~/Library/Application Support/MobileSync/Backup
  
fyicenter$ ls -l 45219035443f09dccbcc469473f487ca5aee9f5a
total 117760
drwxr-xr-x@ 172 fyicenter  staff      5848 Sep  5 23:10 00
drwxr-xr-x@ 168 fyicenter  staff      5712 Sep  5 23:10 01
drwxr-xr-x@ 170 fyicenter  staff      5780 Sep  5 23:10 02
drwxr-xr-x@ 168 fyicenter  staff      5712 Sep  5 23:10 03
...
drwxr-xr-x@ 189 fyicenter  staff      6426 Sep  5 23:10 fd
drwxr-xr-x@ 180 fyicenter  staff      6120 Sep  5 23:10 fe
drwxr-xr-x@ 181 fyicenter  staff      6154 Sep  5 23:10 ff
...
-rw-r--r--@   1 fyicenter  staff    849778 Sep  5 23:10 Info.plist
-rw-r--r--@   1 fyicenter  staff  59351040 Sep  5 23:10 Manifest.db
-rw-r--r--@   1 fyicenter  staff     82927 Sep  5 23:10 Manifest.plist
-rw-r--r--@   1 fyicenter  staff       189 Sep  5 23:10 Status.plist

⇒ Search Files in iPhone Backup on Mac

⇐ Back Up iPhone with iTunes on Mac

⇑ Manage iPhone from iTunes on Mac

⇑⇑ Apple Music and iTunes Frequently Asked Questions